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Furthermore, calcium overload activates calpains, calcium-dependent proteases, which degrade cytoskeletal and membrane proteins, thereby contributing to structural damage and impaired contractility [8]

Cordido F, Penalva A, Peino R, Casanueva FF, Dieguez C

Briefly, pellets containing 30 x 10 6 cells were rinsed with the provided Assay Buffer, sonicated, and centrifuged at 300 x g for 15 min at 4C

Consequently, many proteins must perform more than one task and our structural analysis reveals that this holds for SemD, an effector protein that is involved in the reshaping of membrane structure and actin cytoskeletal organisation during chlamydial endocytosis