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Description

For colony formation assays, cells (5 10 4 ) were suspended in 2 mL of 0.33% noble agar (Difco) and laid over 6-well culture plates previously coated with 2 mL of 0.5% noble agar

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10.1021/acsnano.6b04268 ACS Nano 119 LinertW.KozlowskiH

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Then, IPTG was added to the bacteria to a final concentration of 100 M and incubated at 25 C for 12 h

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