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After the ninhydrin test had indicated complete coupling, the mixture was filtered, and the peptidyl resin was washed three times with DMF

While many of those results may be attributed to GSH depletion, the contribution of GCLC to -glutamyl peptide synthesis and glutamate scavenging may also play a very important role, particularly in the context of xCT inhibition, where cells cannot export glutamate

It also modulates the nitric oxide system, which supports healthy blood circulation and has anti-inflammatory effects

80 These protein complexes play an important role in fibroblast activation in the kidney, and their inhibition reduces or prevents the progression of CKD