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Cells were maintained in a humidified environment at 37C under a 5% CO2 atmosphere for 48 h

illustration for the denitrification and nitrification processes

Research cycles typically run for 4 to 8 weeks, often followed by a break of 2 to 4 weeks before potentially resuming

Dysbiosis, an imbalance in the gut microbial community, is frequently observed in individuals with ASD and is associated with alterations in key neurotransmitters, such as GABA, serotonin (5-HT), and DA, which are crucial for regulating mood, cognition, and social behaviors [179]