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Haemoglobin-bound iron is transported into the intestinal epithelial cells by heme carrier protein 1 (HCP1) that is localised to the intestinal mucosal epithelium

Quantitative PCR was performed using the Roche SYBER Green reagent and a LightCycler 480 apparatus (Roche Diagnostic, Meylan, France) with the following program: one step at 95C for 10 min, 40 cycles of denaturation at 95C for 10 sec, annealing at 65C for 15 sec and elongation at 72C for 15 sec

In 2 women who used them very frequently (up to 3x-4x/hour), coffee enemas caused severe electrolyte imbalances that resulted in death [77+]

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