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Additionally, GSH and GSSG only differ by the presence of a thiol or a disulfide and their molecular weight

10.1159/000176457 274 RathodY

Aliquots (0.5 mL) of the supernatant were mixed with 0.5 mL of 10 mM phosphate buffer (pH 7.0) and 1.0 mL of 1 M potassium iodide, and absorbance was measured at 390 nm