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Description

Most people take it at lunchtime or late morning for best results

Cubillos-Ruiz JR, Bettigole SE, Glimcher LH

Alternatively, for immunoprecipitation or viral production, cells were transfected with polyethylenimine (PEI) (Sigma-Aldrich) in 3:1 PEI:DNA weight ratio

The radioactive proteins synthesized are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and detected by radioautography