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Pursuing this, we performed immunofluorescence staining and detected MOR + signals with a nerve fiber-like morphology in the DMV, which were not co-labeled with the neuronal nuclei (NeuN, a marker for neuron), glial fibrillary acidic protein (GFAP, a maker for astrocyte), ionized calcium-binding adapter molecule 1 (Iba1, a marker for microglia), or a ChAT-specific antibody (Supplementary Fig

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RNA sequencing methods for transcriptome analysis From cryopreserved murine tumor tissues, total RNA was extracted using TRIzol reagent (Invitrogen, CA, USA), and its purity and concentration were measured afterward