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At the time of assay, the growth medium was replaced with HBSS, supplemented with 10 mM HEPES and 0.1% (w/v) ovalbumin and incubated at room temperature for 30 min

In addition, studies have shown that the LC3 II/LC3 I ratio and Beclin-1 expression of the damaged cardiomyocytes increased under the regulation of H 2 -rich water, indicating that H 2 was included in the degradation process of injured mitochondria for intracellular homeostasis (Yao L

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Even small metabolic perturbations can promote reprogramming of cancer cell metabolism, presenting the role of EVs as key in this process (Yang et al., 2020)