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Immunofluorescence staining also showed a marked increase in the number of Ki-67-positive cells and a decrease in the nuclear content of -H2AX and P21 after FOXO4-DRI treatment (Figures 4B,C)

The Centers for Disease Control and Prevention (CDC) and other authorities recommend methotrexate be administered only by healthcare professionals familiar with its uses, toxicities, and safety precautions