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Product Specifications Specification Detail Product typeCell detachment / dissociation reagent FormatSterile-filtered liquid, ready-to-use Volume100 ml Working concentration1x (ready-to-use) Enzyme activityCombined proteolytic and collagenolytic Enzyme originNon-mammalian and non-bacterial Buffer systemDulbeccos PBS with EDTA pH indicatorPhenol red pH range6.8 7.8 AppearanceClear, pale red to orange solution Storage temperature-15 C or below Stability after thawingUp to 2 months at +2 C to +8 C Recommended use volume~10 ml per 75 cm2 culture surface Typical incubation time5 10 minutes at room temperature Shipping conditionsFrozen on dry ice Intended useFor research use and further manufacturing only Formulation (Composition per Liter) Component Concentration (mg/L) Inorganic Salts Sodium chloride (NaCl)8000.00 Disodium hydrogen phosphate (Na2HPO4)1150.00 Potassium chloride (KCl)200.00 Potassium dihydrogen phosphate (KH2PO4)200.00 Other Components EDTA 4Na (tetrasodium EDTA)220.00 Phenol red3.00 Proprietary enzyme blend (proteolytic and collagenolytic activity)1x Accutase is a registered trademark of Innovative Cell Technologies, Inc.75.00*Antibiotic/Antimycotic Solution (100x)Product Overview Volume: 100 ml Storage: -15C Sterility: Sterile-filtered Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications

Current ICH therapies, such as surgical evacuation or corticosteroids, often fail to target molecular mechanisms and may exacerbate secondary injury [43]

No change was observed in Ionized calcium binding adaptor molecule 1 (Iba1), a marker for microglia in the CA1 (Fig

In addition, as myofibroblasts are sensitive to matrix stiffness, developing strategies that reduce the pathological stiffness or alter the mechanical cues of the ECM in a way that deactivates myofibroblasts without disrupting ECM scaffolding needed for fibroblast migration and functional integrity