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Description

Immunity 50 , 10991114 (2019)

Cryo-EM sample preparation and imaging The mTORC1RHEBRAGRagulator4E-BP1 complex on liposomes was reconstituted with the following steps

Cell lines, culture conditions, transfection and infection The Human SimpsonGolabiBehmel syndrome (SGBS) cell line was kindly supplied by Martin Wabitsch (University of Ulm, Germany) 45 and authenticated as preadipocytes based on their response to adipogenic cues (cocktails) and the expression of adipogenic marker genes