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Scratch assay MKN45 or ASPC1 cells were plated in a 6-well culture dish at 5 10 4 cells/dish and grown for 24 h, and the non-migrated cells were scraped off the upper surface of the membrane with a 10 ul pipette

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Complete 4-week protocol example Goal : Heal Achilles tendon injury Protocol : Standard 250mcg twice daily Supply needed : 3x 5mg vials (28 days = 56 doses) 1x 30ml bacteriostatic water 100x insulin syringes Schedule : Morning injection: 250mcg (10 units on syringe) Evening injection: 250mcg (10 units on syringe) Rotate injection sites Track progress weekly Cost breakdown : BPC-157 (3 vials): $75-120 Supplies: $30-50 Total: $105-170 for complete protocol Expected results : Week 1-2: Reduced pain and inflammation Week 3-4: Improved range of motion, functional improvement Post-protocol: Continued healing for 2-4 weeks See our complete injury recovery guide

Its use in humans outside an FDA-authorized clinical trial is illegal