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It was mixed thoroughly on a vortex shaker and further kept for 1 h of incubation at room temperature

In addition, it can be suggested that the elevated glutamate uptake capacity in the astrocytes might be in response to increased glutamate shuttling form neurons which possibly release the excess glutamate that is not incorporated in the TCA cycle due to hampered GDH levels

This dysregulation orchestrates a symphony of cellular responses, including activation of oxidative stress, impairment of the ubiquitin-proteasome system and activation of protein toxicity stress responses, all of which converge to regulate cell death

The procedure is quick and involves a simple injection into the upper arm or thigh