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A Luminex flow cytometer (RRID: SCR_018025) distinguished the antigens by their bead color and quantified the amount of antibody via the level of fluorescence, reported as median fluorescence intensity (MFI)

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Second, some DAMPs (such as HSP70) can directly act on the TLR2/4 receptors on the surface of T cells, regulating T cell function through a dual mechanismpromoting the proliferation of effector T cells while inhibiting the immunosuppressive activity of regulatory T cells (Tregs)