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Although serine can be derived from the degradation of cellular proteins and the interconversion with glycine, these pathways alone are insufficient to meet the metabolic demands of biological tissues, especially in pathological conditions ( de novo serine synthesis pathway (SSP) substantially enhances the intracellular availability of serine ( The de novo serine synthesis pathway, also referred to as the serine biosynthetic pathway, is a branch pathway of glycolysis

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As revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and bicinchoninic acid (BCA) assays, iron deficiency resulted in enhanced accumulation of mitochondrial misfolded proteins (Fig