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The MAPK MEK1/2-ERK1/2 pathway and its implication in hepatocyte cell cycle control

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Both assays are based on the enzymatic oxidation of L- or D-lactate to pyruvate by L-lactate dehydrogenase (L-LDH) or D-lactate dehydrogenase (D-LDH), respectively, coupled to the reduction of NAD + to NADH (Equation 1), which was quantified spectrophotometrically at 340 nm