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The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 g/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 g/mL) (Bio-Sciences) for 30 min protected from light, at room temperature

The PepT1 targeting mechanism means KPV's oral GI effects are dependent on having IBD-like PepT1 upregulation in the colon

Bruising is more likely if: A needle is too large The injection angle is off The needle is pulled out too quickly The injection is done too fast There is less fat under the skin, which means less padding Certain medications or supplements are being used, such as blood thinners, fish oil, or vitamin E How to prevent bruising Use a 29 to 31 gauge insulin syringe with a 4 to 8 millimeter needle