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Controlled human trials exist, but they're limited in size, focused mostly on topical cosmetic use, and usually last only a few weeks or months. That means a lot of the exciting claims, especially around gene expression, systemic repair, and anti-aging effects, haven't been confirmed in large-scale human studies

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Nearly two-fifths of the US population has a marginal amount of vitamin B12 (cobalamin) in their bodies, consequently putting them at a high risk of a deficiency

The structural difference is a single amino acid substitution at position 1: glycine in GHK-Cu and alanine in AHK-Cu, which is thought to shift the peptide's biological affinity more specifically toward hair follicle cells rather than the broader tissue-repair profile of GHK-Cu