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This process is morphologically marked by small mitochondria with condensed membrane densities, and biochemically by iron overload and GSH depletion, leading to loss of antioxidant defense and irreversible oxidative damage ( 3.2 Key molecular drivers of ferroptosis Iron Overload: Excess intracellular ferrous iron (Fe 2+ ) catalyzes the Fenton reaction, producing hydroxyl radicals (OH) that initiate lipid peroxidation cascades

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The 96-well plate was gently shaken for a few seconds and absorbance was read at 340 nm once every minute for 10 min, or to obtain readings at a minimum of 5 time points

A meta-analysis of OLP patient data revealed that smokers had a significantly greater rate of malignant transformation than nonsmokers (Aghbari et al., 2017)