glutathione column purification GST-tagged Protein Kit columns and binding Separation with Cytiva Products (GST – carbon spacer arm Glutathione Agarose Resin 6FF for

doi: 10.1136/gutjnl-2022-328403 13 BajajJ

Water, Dipropylene Glycol, Galactomyces Ferment Filtrate(50,029 ppm), Glycerin, Methylpropanediol, Glycereth-26, Niacinamide, Glyceryl Glucoside, Glyceryl Polymethacrylate, Erythritol, Polyglyceryl-10 Laurate, Hydroxyacetophenone, 1,2-Hexanediol, Carbomer, Caprylyl Glycol, Arginine, Hydrolyzed Jojoba Esters, Xanthan Gum, Adenosine, Glucose, Ethylhexylglycerin, Butylene Glycol, Citric Acid, Ascorbic Acid, Polyquaternium -51, Panthenol, Citrus Aurantium Bergamia (Bergamot) Fruit Oil, Diethylhexyl Sodium Sulfosuccinate, Sodium Citrate, Lavandula Angustifolia (Lavender) Oil, Glutathione(10.89 ppm), Sodium Hyaluronate, Dipotassium Glycyrrhizate, Oryza Sativa (Rice) Extract, Pyrus Malus [Apple] Fruit Extract, Saccharide Isomerate, Hydrogenated Lecithin, Pantolactone, Maltodextrin, Brassica Oleracea Italica (Broccoli) Extract, Hydrolyzed Vegetable Protein, Prunus Serrulata Flower Extract, Nelumbo Nucifera Flower Extract, Lilium Tigrin um Extract, Copper Tripeptide-1, Hydrolyzed Hyaluronic Acid, Hyaluronic Acid, Sodium Hyaluronate Crosspolymer, Hydroxypropyltrimonium Hyaluronate, Potassium Hyaluronate, Hydrolyzed Sodium Hyaluronate, Pentylene Glycol, Sodium Acetylated Hyaluronate, Disodium EDTA, Limonene, Linalool

The K m value for CDNB was obtained by reacting 0.5 g of purified e-GSTs of mammals with variable amounts of CDNB (from 0.05 to 2 mM) in the presence of 1 mM GSH, in 0.1 M potassium phosphate buffer, pH 6.5 (25 C)

PMID: 220 SatoKHallCGlaserSFrancisHMengFAlpiniG