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After overnight incubation, the medium was replaced with Williams E Medium supplemented with primary hepatocyte maintenance supplements (Thermo Fisher Scientific, catalog: CM4000) and incubated for 24 h before compound treatment

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In case of acetaminophen toxicity, acetylcysteine is usually administered as 70 mg/lb (140 mg/kg) firstly as a loading dose of acetylcysteine, which is orally or intravenously administered

Furthermore, rule-shift learning, a form of cognitive flexibility, was improved by chronic NAC-treatment in the CD2-KO group due to a lower rate of perseverative and random errors, compared to untreated knockouts