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Cell imaging and analysis Thirty minutes before imaging, cells were rinsed with 37 C ERB buffer (120 mM NaCl, 3.5 mM KCl, 1.2 mM CaCl 2 , 1 mM MgCl 2 , 0.4 mM KH 2 PO 4 , 5 mM NaHCO 3 , 1.2 mM NaSO 4 , 20 mM HEPES, 5 mM Glucose, pH 7.4 and adjusted at 290 mOsm)

Bidirectional control of generalized epilepsy networks via rapid real-time switching of firing mode