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Indeed, we detected weak ZSWIM8 binding to RNA alone in filter-binding experiments, with affinity increasing as RNA lengths increased from 28 to 120 nt (Extended Data Fig

Whatever your protein source is, youll want to load it onto the column in pretty much the same way cap the bottom, add your interacting protein or cell lysate, cap the top, and incubate with gentle agitation for around an hour

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Light microscopy may also be used to make this distinction