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The organoids were incubated with dispase at 37 C for 30 min, after which they were washed twice with cold DMEM/F12 and collected by centrifugation (5 min, 4 C, 300 g )

4 This means glutathione may help protect cells from damage caused by free radicals

Expression analysis of genes involved in iron metabolism and ferritinophagy indicated that the Auranofin and combination groups could be promoting this process (Fig