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On an experimental day, cells were washed with Hanks Balanced Salt Solution (HBSS, Gibco/ThermoFisher Scientific) and the respective receptors were labeled with 100 nM HaloTag NanoBRET 618 ligand (Promega Corporation, Fitchburg, WI) in phenol red-free Opti-MEM I reduced serum medium (Gibco/ThermoFisher Scientific) (2 h, 37 C, 5% CO 2 )

The pathological mechanisms and potential therapeutic drugs for myocardial ischemia reperfusion injury

An online parallel memory Lagrangian tracking algorithm was used to simulate observed drifter trajectories

The correlations of the subscales of sensation, social interaction, language, and self-care with these indicators were similar, with only creatinine showing a positive correlation, while the others were all negatively correlated