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To examine the agonist activities of HGF mutants, naive H1299 cells were plated 200,000 cells/well on six-well plates (Corning) and starved in an FBS-free medium for 16 h

Thereafter 34 mice were treated every 24 h for three successive days with either saline alone (control), saline with IL-13, saline with GGsTop, or saline with IL-13 and GGsTop

Mice were followed for 28 days for sciatic function index (SFI), and sciatic nerves and hind limb muscles were harvested for histomorphological and cellular analyses