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The reaction yields a colorimetric signal where samples with reduced amounts of SOD emit a lesser signal that can be measured via colorimetry ( Catalase CAT is quantified by adding hydrogen peroxide (H 2 O 2 ) to the sample and analyzing the rate of decomposition of CAT, which is proportional to a reduction in the absorbance reading generated by the instrument ( Reduced Glutathione GSH can be determined by adding 5,5-dithiobis-2-nitrobenzoic acid (Ellman's reagent) to the sample

Also, CML levels correlated with severity of disease [99]

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