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Primary antibodies used were: Phosphorylated P70S6 Thr389 Kinase (Cat# 9205 (also 9205L, 9205S), RRID:AB_330944), Total P70S6 Kinase (Cat# 9202 (also 9202L, 9202S), RRID:AB_331676), Phosphorylated 4EBP1 Thr37/46 (Cat# 2855 (also 2855L, 2855S, 2855P), RRID:AB_560835), Total 4EBP1 (Cat# 9644 (also 9644S, 9644P), RRID:AB_2097841), Phosphorylated AMPK Thr172 (Cat# 50081, RRID:AB_2799368), Total AMPK (Cat# 2532 (also 2532L, 2532S), RRID:AB_330331), Phosphorylated AKT Thr308 (Cat# 9275, RRID:AB_329828), Phosphorylated AKT Ser473 (Cat# 9271 (also 9271S, 9271L, NYUIHC-310), RRID:AB_329825), Total AKT (Cat# 4691 (also 4691L, 4691P, 4691S), RRID:AB_915783), Phosphorylated ERK Thr202/Tyr204 (Cat# 4370 (also 4370L, 4370S, 4370P, 4370T), RRID:AB_2315112), Total ERK (Cat# 4695 (also 4695P, 4695S), RRID:AB_390779), and LC3A/B (Cell Signaling Technology Cat# 4108 (also 4108S), RRID:AB_2137703), all from Cell Signaling Technologies (Danvers, MA, USA)

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Rheumatology (Oxford)