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These symptoms include: an itchy red rash that includes hives or welts swelling in the throat or possibly other areas of the body wheezing, coughing, or difficulty breathing tightness of the chest difficulty swallowing vomiting diarrhea cramping of the stomach pale or red color of the face or body Stevens-Johnson syndrome Stevens-Johnson syndrome is a rarer complication of sulfa allergy

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After subsequent washing, 50l/well of 1:1000 diluted streptavidin-ALP (Mabtech) was added, incubated for 1 hour, and then developed with 50 l/well of AP Conjugate sub kit (BioRad) for 510 minutes