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doi: 10.1182/bloodadvances.2020002944 110 LouissaintAJr.SchafernakKTGeyerJTKovachAEGhandiMGratzingerDet al

In this chapter, we are describing methods to recombinantly express and purify galectins using three different methods of affinity purification, i.e., lactosyl-Sepharose chromatography for fungal galectin Coprinopsis cinerea galectin 2 (CGL2), nickel-chromatography for histidine-tagged human galectin-7, and glutathione-Sepharose chromatography for Glutathione S -transferase-tagged (GST-tagged) human galectin-7