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Isothermal titration calorimetry Experiments were carried out on a MicroCal auto-ITC200 instrument at 25 C while stirring at 750 rpm in the ITC buffer of pH 6.5, consisting of 100 mM sodium phosphate, 2 mM EDTA and 2 mM -mercaptoethanol

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In addition, Glu can act as a long-distance signaling transducer among cells, tissues, organs, and even the whole plants by the crosstalk with Ca 2+ , ROS, and electrical signaling (Mousavi et al., 2013