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Adenosine triphosphate (ATP) detection Total cellular ATP levels were determined using a bioluminescent ATP assay kit (Beyotime)

Cells were fixed with 90% ice-cold ethanol with gentle vortexing and incubated on ice for 1 h

Table 3 Application related forms of ICD

Why Your Doctor Might Not Have Diagnosed It Many Chicago women tell me their doctor said theyre too young for menopause or ran a single hormone test that came back normal. Heres the problem: standard medical training focuses on menopause (when hormones are consistently low) and doesnt adequately address perimenopause (when hormones are fluctuating wildly)