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Post-infection, virus was removed and cells were selected with puromycin (lentiCRISPR-v2), blasticidin (pMXS_IRES_Blast) or by flow sorting of top 1% GFP+ cells (lentiCRISPR-v1(GFP))

Kotis and his Arlington Heights team will provide you with detailed instructions prior to surgery, but here is a general overview of what to expect: Wear your compression garment as directed to support your new contours Walk lightly as soon as you can to prevent blood clots and promote healing Any pain or discomfort can be managed with light walking and medication Swelling, bruising and numbness should begin to subside in about a week Most patients can return to sedentary work in about a week Some residual swelling may persist for several months Dr

EMBO Mol Med 9:571588

"Finding that interesting loop later led to our understanding of the sensing mechanism," Birsoy says