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Since miropsin-1 and miropsin-2 exhibited low activity to the protein substrates, we examined whether these two serine proteases were active against several synthetic amino acid chromogenic substrates with p-nitroaniline ( p NA) as a leaving group including MeoSuc-AAV- p NA, Suc-AAPF- p NA, Suc-AAPL- p NA, Suc-AAPA- p NA, p-Tosyl-GPK- p NA, and Suc-AAPR- p NA

This population of E3 ligase is primed for the target recruitment and is likely to persist inside the cell 40,41,42