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population was at risk for anemia (low red blood cells or hemoglobin, often caused by iron deficiency) or at least one vitamin deficiency---meaning they fell below the deficiency cutoffs established from CDC data for at least one micronutrient

Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPX3 and their serial dilutions

2.13 Statistical analysis Primer 3 1 was used to design primer sequence (Supplementary Table S1)