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In these experiments, the thermal denaturation of the proteins is followed by monitoring the fluorescence enhancement of a probe (SYPRO Orange) that binds to protein hydrophobic patches upon denaturation process in the presence or absence of chemical compounds

10.1142/S0192415X15500664 8 EntezariM.HashemiD.TaheriazamA.ZabolianA.MohammadiS.FakhriF.et al (2022)

(PubMed) Wang Y, Jin Y, Wang Y, et al

DSIP offers historical human data and subcutaneous administration but suffers from an undefined mechanism, inconsistent clinical results, and decades of research that have failed to identify its specific pharmacological target