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Confirming our previous results, each of the mutations R 392 A, F 471 A or Y 473 A, abolished the TRAF6 interaction with LMP1 in HEK293 cells, whereas the K 469 A mutation had no negative effect on the interaction between the two proteins

After 2 days of culture (approximate doubling time of the cells), the cell monolayer was scratched to make a wound using a plastic micropipette tip, which was performed in the wound healing assay

Hence this supplementary approach should not be considered for this purpose until this potential role been further elucidated