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The difference likely relates to the specific conformational changes each agonist induces in the receptor, which determines which G-protein pathways are preferentially activated

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The inclusion bodies were washed with 25 ml of wash buffer (100 mM Tris pH 8.0, 2 mM EDTA and 0.01% v/v deoxycholate), sonicated and pelleted by centrifugation

In addition to promoting vasodilation, under high sodium culture conditions or high salt stimulation, capsaicin significantly inhibited the proliferation of rat aortic smooth muscle cells in a dose dependent manner, accompanied by upregulated TRPV1 expression (134)