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The images were acquired as stacks of ~40 slices with at 0.75 m z-stack step size intervals using a x40 (NA 1.3) oil immersion objective, which were later combined to obtain a single high-quality image displaying detailed magnification of the cell processes

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Behavioral results also indicate that inhibition of caspase-3 activity by both pharmacological therapy with caspase-3 inhibitor Z-DEVD-FMK and caspase-3 knockdown attenuates the thermal hyperalgesia in CCI rats (Wu et al., 2012)