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Membranes were blocked with 5% skimmed milk in Tris-buffered saline containing 0.05% Tween 20 for 1 h at room temperature before overnight incubation at 4C with a 1:1,000 dilution of primary antibodies against total and phosphorylated AMPK and total and phosphorylated ACC (cat

This documentation offers researchers complete transparency and confidence in the quality of the product they are using in their laboratory investigations