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After the lung extracts were centrifuged (12,000 g , 10 min at 4C), the supernatant was collected, and protein concentrations were determined using a Bicinchoninic Acid (BCA) Protein Assay Kit (Beyotime Institute of Biotechnology)

However, B16/BL6 melanoma cells produce soluble CD155 to suppress the DNAM-1-mediated degranulation of NK cells against tumor cells, creating an immunosuppressive environment that facilitates lung colonization [200]

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