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In mammals, LC treatment activated the main marker proteins of mitophagy, such as PINK1, Parkin, BCL2/adenovirus E1B 19 kDa protein interacting protein 3 (BNIP-3) and microtubule-associated protein 1 light chain 3 B-II (LC3B-II) (Reference Sabry, Ahmed and Maksoud73,Reference Choi, Ohn and Jung75) , and decreased hepatic 8-hydroxydeoxyguanosine in rats (Reference Chang, Nishikawa and Nishiguchi79)

Although early research shows promise, it remains largely experimental, and long-term safety data is still limited

A yeast two-hybrid assay (Y2H) was performed to confirm the interaction between CNMT and TFR1, with the T + p53 group as a positive control (Fig