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For laboratories conducting in vitro or specialized in vivo studies requiring liquid solution: Diluent: Use deionized water or a physiological saline solution for solubility

NAD can also be generated de novo from the essential amino acid tryptophan, essentially in the liver, but it is a less efficient precursor as 60 mg of Trp is considered the equivalent of 1 mg of niacin [67]

Briefly, the antibodies were cross-linked to Protein A Sepharose beads with dimethyl pimelimidate (Sigma) and lysates were incubated overnight with each antibody