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After incubation, the bacteria were again washed twice with PBS following low-temperature centrifugation

While the role of glutathione and specific enzymes is well understood in other types of abiotic and biotic stresses, particularly those associated with bacteria or fungi, recent advances in research have highlighted the significance of glutathione modulation and mutations in genes encoding glutathione-associated enzymes in triggering immunity or susceptibility against plant viruses

GCL is considered the speed limiting enzyme of synthesis since overexpression of GS does not increase GSH levels while overexpression of GCL increases the synthesis of GSH (61) (Figure 3)

Oncotarget 8(17):2806328073 Hayano M, Yang WS, Corn CK et al (2016) Loss of cysteinyl-tRNA synthetase (CARS) induces the transsulfuration pathway and inhibits ferroptosis induced by cystine deprivation