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2.2 Cell culture and analysis of cell proliferation Lymphoblasts were grown in RPMI 1640 medium from Sigma-Aldrich (Saint Louis, MO, USA, R6504) supplemented with 2 g/L sodium bicarbonate, 15% (v/v) fetal bovine serum and 0.5% (v/v) of penicillin-streptomycin plus amphotericin B in T25 or T75 flasks, in an upright, at 37C in a humidified atmosphere of 5% CO 2

Dissociable roles of prelimbic and infralimbic cortices, ventral hippocampus, and basolateral amygdala in the expression and extinction of conditioned fear

Consistent with this, GLP1R protein is detected in -cells but not in - or -cells, despite the presence of its mRNA (Gray et al., 2020)