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Isolation, Purification, and Structure Elucidation of Unknown-1 Metabolite Multiple incubations of 7-HMG with human plasma were necessary to obtain a sufficient amount of unknown-1 for analysis using fractionation, preparative thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) techniques

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Glutamate receptor-mediated analgesic activity of NAC was first observed in rodent models [74,75] and this same metabotropic glutamate receptor-mediated analgesic effect of NAC was later demonstrated in human volunteers exposed to laser stimuli pain [76]