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These changes can cause oxidative modifications to reactive thiols of structurally- or functionally-critical proteins, such as transcription factors 43 , signal transduction molecules 44 or metabolic enzymes 45 , and thereby evoke cellular defense mechanisms against oxidative damage 46

Diabetologia 53 , 17221726 (2010)

32 Efflux lowers the intracellular drug concentration below inhibitory levels

2 Dual-column LC-HRMS data acquisition Each sample is run in two separate LC-HRMS acquisitions: reversed-phase (RP) chromatography for non-polar and moderately polar metabolites, and hydrophilic interaction liquid chromatography (HILIC) for highly polar species (amino acids, nucleotides, organic acids, sugars)