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DOI: 10.1016/j.cmet.2009.05.006 McKenna, N.J., Cooney, A.J., DeMayo, F.J., Downes, M., Glass, C.K., Lanz, R.B., Lazar, M.A., Mangelsdorf, D.J., Moore, D.D., Qin, J., Steffen, D.L., Tsai, M.J., Tsai, S.Y., Yu, R., Margolis, R.N., Evans, R.M., O'Malley, B.W

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Briefly, muscles were washed in chilled PBS and immediately transferred to 30% sucrose and kept at 4 C until tissue sank to bottom

As concerns other micronutrients, deficiencies of selenium and/or iron or zinc would increase the detrimental effect of iodine deficiency on thyroid function, and/or impair antioxidant defences of thyrocytes [54,55,56,57]