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In the presence of AGE-MGO, the translocation of Nrf2 from the cytosol to the nucleus is inhibited, which results in decreased expression of detoxifying enzymes such as heme oxygenase-1(28)

Compared to FACS, our GST affinity cell sorting system does not require expensive equipment and is less likely to cause mechanical cell damage ( The recently reported positive cell sorting system utilizes GPI-anchored Twin-Strep-Tag as the affinity tag and allows the positive cells to be enriched with Strep-Tactin magnetic beads ( Reduced GSH is widely expressed in plant, animal, and fungal cells ( In brief, we develop a gene transfection-positive cell sorting system utilizing GPI-anchored GST as the affinity tag that enables positive cell isolation using GSH magnetic beads

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This classically produces urticaria, characterized by transient, intensely pruritic wheals, or anaphylaxis